Association Of Nitrogen-Fixing Bacteria In Rice

Authors

  • Muhammad Muhammad Universitas Darussalam Gontor https://orcid.org/0000-0002-3885-2730
  • Liliek Sulistyowati Faculty of Agriculture, University of Brawijaya Malang
  • Wiwik E Widayati Research Staff of the Indonesian Sugar Plantation Research Center
  • Parwito Parwito Departement of Agrotechnology, Faculty of Agriculture, Universitas Ratu Samban, Bengkulu

DOI:

https://doi.org/10.37638/sinta.3.2.49-56

Abstract

The research was aimed first: to understandability of GFP marker (green fluorescent protein) gave identities nitrogen-fixing bacteria that clear as endophytic when was the application to plant tissue. The research was aimed second: to understandability of nitrogen-fixing bacteria make special colonization as endophytic use Seed dressing method and Dipping method. The results of the research are GFP markers that show its ability to provide a clear identity as an endophytic bacterium when applied in natural microbial communion. Besides that, GFP is also stable in the applications on rice plant tissue and is able to provide a fairly clear expression. The bacteria Azospirillum lipoferum-1224, Azospirillum lipoferum-1247, Azorhizophilus paspali-2283, Azotobacter chroococum-2286 were able to colonize the rice plant tissue using either the inoculation method of Dipping or Seed dressing.

References

Dobereiner, J., V. L. D. Baldani dan V. M. Reis, 1995. Endophytic Occurrence of Diazotrophic Bacteria in Non-Leguminous Tree. NATO ASI Series, Vol G37 : Azospirillum and Related Microorganism. Springer-Verlag Berlin Heidelberg, Berlin. pp : 3 -14. Dalam Dong, Z., M. E. McCully, & M. J. Canny. 1997. Does Acetobacter diazotrophicus Live and Move in The Xylem of Sugarcane Stem : Anatomical and Physiological Data. Annals of Botany. 80 : 147 – 158

Dong, Z., A. L. Inguez dan E. W. Triplett, 2003. Quantitive Assessment of The Host Range and Strain Specificity of Endophytic Colonization by Klebsiella pneumonia Kp342. Plant and Soil. 257 : 49 – 59

Gyaneshwar, P. James, E. K. Mathan, N. Reddy, P.M. Reinhold-Hurek dan J.K. Ladha, 2001. Endophytic colonization of rice by a diazotrophic strain of Serratia marcescens. Journal of Bacteriology, Apr. p.2634-2645.

Kamuru, F., S.L. Albrech, L.H. Allen, K.T. Shanmugan, 1998. Dry matter and nitrogen accumulation in rice inoculated with a nitrogenase-derepressed mutant of Anabaena variabilis. Argon. J: 529-535.

Kennedy, I.R. Lyli, L. Pereg-Gerk, C. Wood, R. Deaker, K. Gilchrist dan S. Katupitiya, 1997. Biologikal nitrogen fixation in non-leguminous field crops. Fasilitating the evolution of an effective association between azospirillum and wheat. Plan and soil 194: 65-79

Lagreid, M., O.C. Bokman dan O. Kaarstad, 1999. Agriculture, Fertilizers, and Environment. CABI Publishing in association with nork Hydro ASA. 294; 112-149.

Mano, H. dan H. Morisaki, 2008. Minireview. Endophytic bacteria in the rice plant. Microbes Environment. 2(23) : 109-117.

Rosenblueth, M. dan E. Martinez-Romero, 2006. Bacterial Endophytes and Their Interactions with Hosts. 19 (8) : 827-837

Syamsudi, C. R. S., 1997. Penyakit Dalam Simpanan (penyakit gudang). Lembaga Penerbitan FP Universitas Brawijaya. Malang. p. 66

Tsien, R.Y., 1998. The green fluorescent protein. Annu Rev Biochem 67:509–544

Watanabe, 1986. Nitrogen Fixation By Non-legumes in Tropical Agriculture With Special Reference to Wetland Rice. J. Plant and Soil. 90 : 343 – 357.

Widayati, W.E., 2005. Bakteri Diazotrof endofit Pada Tebu: Identifikasi dan Mekanisme Asosiasi. Disertasi Program Bioteknologi, Universitas Gadjah Mada Yogyakarta. 219: 41-46

Downloads

Additional Files

Published

2022-12-31

Issue

Vol. 3 No. 2 (2022)

Section

Articles