Single spore culture of Arbsucular Mycorrhizal Fungi from the Rhizosphere of Siompu Citrus (Citrus nobilis Lour.)
DOI:
https://doi.org/10.37638/sinta.5.1.47-54Abstract
Arbuscular mycorrhizal fungi (AMF) are the most common type of mycorrhiza and are the result of an obligate symbiont between fungi and roots that form a mutualistic symbiosis in several plants. This research aims to determine the increase in AMF spore production from the rhizosphere of Siompu oranges using single culture at the Nggula-nggula location. This research was conducted at the Plastic House of the Indonesian Mycorrhizal Association (AMI) Southeast Sulawesi Branch, UHO Old Campus and the UHO Forestry and Environmental Science Laboratory Unit which lasted for 3 months from May – July 2023. This research was designed using a Completely Randomized Design consisting of 12 treatments, each treatment was repeated 3 times and 5 plants were used for each repetition so that the total plants were 180 plants. The results showed that AMF spore production was not influenced by the type of AMF treatment. The types of spores produced in a single spore culture range from 13-127 spores at 3 months of age.
References
Asmarahman, C., S.W. Budi., I. Wahyudi, I dan E. Santoso. 2018. Identifikasi Mikroba Potensial Fungi Mikoriza Arbuskula (FMA) Pada Lahan Pascatambang PT. Holcim Indonesia Tbk. Cibinong, Bogor, Jawa Barat. Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management). 8(3): 279-285.
Breugel M.V., J.S. Hall, D.J. Craven, T.G. Gregoire, A . Park, D H. Dent, M.H. Wishnie, E. Mariscal, J. Deago, D. Ibarra, N. Cedeno dan M.S. Ashton. 2011. Early Growth and Survival of 49 Tropical Tree Species Across Sites Differing in Soil Fertility and Rainfall in Panama. For Ecol Manag. 261: 1580-1589.
Guritno. B. 1995. Pertumbuhan tanaman. UGM Press. Yogyakarta.
Husna, Mey D, dan Yulistiati, T. 2004. Pengaruh Inang dan Media Tanam Terhadap Perbanyakan Spora CMA Asal Muna dan Kendari. Agriplus. 14(3): 193-198.
Husna., S.W. Budi, I. Mansur, C. Kusmana dan K. Kramadibrata. 2014. Fungi Mikoriza Arbuskula pada Rizosfer Pericopsis mooniana (Thw.) Thw di Sulawesi Tenggara. Jurnal Berita Biologi. 13(3):263-273.
Husna. 2015. Potensi Fungi Mikoriza Arbuskula (FMA) lokal dalam konservasi ex-situ jenis terancam punah kayu kuku [Pericopsis mooniana (Thw.) Thw].
Husna., F.D. Tuheteru dan E. Wigati. 2017. Growth Response and Dependency of Endangered Nedun Tree Species (Pericopsis mooniana) Affected by Indigenous Arbuscular Mycorrhizal Fungi Inoculation. Journal Nusantara Bioscience. 9(1):57-61.
Kafid, M., L.Q, Ain dan B, Prasetya. 2015. Peran mikoriza arbuskula dan bakteri Pseudomonasfluorescens dalam meningkatkan serapan P dan pertumbuhan tanaman jagung pada andisol. Jurnal Tanah dan Sumberdaya Lahan. Vol. 2(2):191-197.
Karepsina, S. 2007. Keanekaragaman Fungi Mikoriza Arbuskula dari Bawah Tegakan Jati Lokal (Tectona grandis Linn. F) dan Potensi Pemanfaatannya [tesis]. Bogor. Institut Pertanian Bogor.
Muryati, S., I.Mansur dan S.W. Budi. 2016. Keanekaragaman fungi mikoriza arbuskula pada rhizosfer Desmodium spp asal Pt. Cibaliung sumberdaya Banten. Jurnal Silvikultur Tropika. 7(3):188-197.
Nusantara, A.D. 2011. Pengembangan Produksi Inokulan Fungi Mikoriza Arbuskula Berbasis Bahan Alami dan Pemanfaatannya Untuk Produksi Bibit Jati (Tectona grandis L.F).Sekolah Pasca Sarjana Pertanian Bogor. Bogor.
Nusantara, A.D., Y.H. Bertham dan I. Mansur. 2012. Bekerja dengan fungi mikoriza arbuskula. Seameo Biotrop. Bogor.
O’Connor PJ, Smith SE, Smith FA. 2001. Arbuscular mycorrhizal associations in the southern southerm simpson desert. 49(1): 493 – 499.
Prayudyaningsih, R dan R. Sari. 2016. Aplikasi Fungi Mikoriza Arbuskula (FMA) dan Kompos Untuk Meningkatkan Pertumbuhan Semai Jati (Tectona grandis Linn. f.) pada Media Tanah Bekas Tambang Kapur: The Application of Arbuscular Mycorrhizal Fungi (AMF) and Compost to Improve the growth of Teak Seedlings (Tectona grandis Linn. f.) on Limestone Post-mining Soil. Jurnal Penelitian Kehutanan Wallacea. 5(1): 37-46.
Samsi, N dan Y.S. Pata’dungan. 2017. Isolasi Dan Identifikasi Morfologi Spora Fungi Mikoriza Arbuskula pada Daerah Perakaran Beberapa Tanaman Hortikultura di Lahan Pertanian Desa Sidera. Agrotekbis: E-Jurnal Ilmu Pertanian. 5(2): 204-211.
Satria, N., Wardati dan A. Khiri. 2015. Pengaruh pemberian kompos tandan kosong kelapa sawit dan pupuk NPK terhadap pertumbuhan bibit tanaman gaharu (Aquilaria malaccensis). Fakultas Pertanian, Universitas Riau. Pekanbaru.
Smith SE, Read DJ. 2008. Mycorrhizal Symbiosis. Third ed. New York (US): Academic Press.
Sianturi, R. P. Delvian, D dan Elfiati, D. 2015. Keanekaragaman Mikoriza arbuskula (FMA) pada beberapa tegakan di areal arboretum Universitas Sumatera Utara. Peronema Forestry Science Journal. 4(2): 128-138.
Souza, T. 2015. Handbook of Arbuscular Mychorrhizal Fungi Springer. New York.
Tuheteru, F.D. 2003. Aplikasi Asam Humat Terhadap Sporulasi CMA dari Bawah Tegakan Alami Sengon [Paraserianthes falcataria (L.) Nielsen] Asal Maluku [skripsi]. Institut Pertanian Bogor. Bogor
Wang, J., G.G. Wang., B. Zhang., Z. Yuan., Z. Fu., Y. Yuan., L. Zhu., S. Ma and J. Zhang. 2019. Arbuscular mycorrhizal fungi associated with tree species in a planted forest of eastern china. Mdpi. 10: 1–14
